Hi Sharda,

Red PCR staining is to localize the orientation of collagen fibres (a reprint enclosed Figure 8). You already know Mason Trichrome.

If you try carefully, you can do using this Mason Trichrome protocol. I used it and you do not need kit but you have to make some solutions very carefully and follow the protocol carefully. If you have any question you may ask me. Also I am enclosing a paper I used MT protocol and see Figure 2. There is one more paper enclosed where I did PSR and did polarized microscopy in Figure 8 on that for PSR

Any question, le me know,

Good luck

Subhash

Subhash C. Juneja Thank you sir for your guidance. I will follow the protocol and in case need any help I will ask you.

For me one of the best staining method for collagen fibres is the Masson's trichrome, Goldner's variant with Green light in place of Anilin blue. So collagen fibres appear in pure green color. Below 2 ex. Regards

Hi Sharda,

Only picrosirius red is not enough for collagen detection, but:

picrosirius red + polarization (=combined with polarisation microscope) method

is very good.

best regards

Peter

Mason trichrome and Verhofe

Mohamed Mahdy I have to use it to measure collagen fibre formation and collagen deposition during the wound healing process

You can use the following stains:

Mallory's trichrome

Crossmon's trichrome

Van Gieson stain

Trichrome Masson, so the collagen will be hilighted in blue or light green with Goldner.