I want to determine if in two lignocellulosic biomass samples, one contains more acetylated polysaccharides than the other. Ideally I shouldn't use more than 20mg per rep.
Ricardo,
there exists a simple smimicro-method for determinatiom of acetyl groups in wood and related materials. Its principle is simple and dwells in the sample deacetylation in an aqueous solution of /COOH/2 in the presence of IS /propionic acid/. Deacetylation proceeds at elevated temperatures /152 oC/ in small glass pipes with by flame sealed ends /after weighing the sample preliminarily pulled capilary end/. Acetic aid is determined via GLC, and amount of acetyl in the material analyzed is calculated from the amount of determined CH3COOH. and oven dry weight of the sample.
The whole analyses incl. its conditions is described in:
R. Solár, F. Kačík and I. Melcer: Simple semimicro method for the determination of O-acetyl groups in wood and related materials. Nordic Pulp and Paper Research Journal, No 4, 1/1987, p. 139 - 141.
Note: You can download the paper from RG page of Rastislav Solár /"contributions"/.
R.S.
Thanks Professor Solár!
In the end I found another method, which I think I will follow: Manabe et al., 2011. Loss-of-Function Mutation of REDUCED WALL ACETYLATION2 in Arabidopsis Leads to Reduced Cell Wall Acetylation and Increased Resistance to Botrytis cinerea. Plant Physiology, 155: 1068-1078.
I do realise it is not such a powerful method as the one you've recommended; however, it will suffice for the type of analysis I am doing.
Much appreciated!
It is OK, you are free to use any method you can Ricardo. Thanks for up-voting.
Best regards,
R.S.
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