For my thesis, I should use (inVitro ) epoprostenol (Flolan) as platelet inhibitory drug, but I did not find the appropriate dose and method to adding Flolan after washing the platelet to preparing the sample for western blotting.
To test the inhibitory effect of any molecule on platelets, the PRP must be incubated (30 min, for example) with different concentrations to determine the IC50.
Once you determine the optimum concentration, you can make subsequent manipulations.
Basma may I ask you to tell me your Idea about the results of this article. Based on your recommend the best concentration is 1 nM of epoprostenol? page 3 (448)