CyQuant cell prolif. assay - how old can it be and still work??

Hi, I have frozen two 96-well plates with cells that I need to stain using the CyQuant Cell Proliferation Assay from ThermoFisher (C7026). However, I didn't realize that we only have some very old...

06 January 2021 244 4 View

Variable with negative values in SPSS?

Hello, dear colleagues! I would ask you for a little help. Actually, I'm doing a multivariate linear regression analysis in the SPSS and one of the independent variables has negative values. In...

20 December 2020 535 2 View

Subgroup analysis in small numbers allowed?

Hello, I had a question regarding subgroup analysis in small patient numbers. I am currently writing a paper on very rare tumours and the response to therapy in a real-world cohort, so no...

18 March 2020 4,983 16 View

Hot to test differences of mean(T test) on all variables in overlapping samples?

Good afternoon, I am working on my thesis on team work and I need to compare if teams differ when I adopt the rule 'at least 2 members of the team gave valid data' (19 teams in total), against...

14 July 2019 5,957 5 View

How do I Obtain Permission to administer the Servant Leadership Questionnaire?

I need to use the SLQ for my dissertation studies.

08 August 2018 1,864 3 View

Searching for articles on experimental power role manipulation in social psychology, can you recommend me some literature?

Hello everyone, Since I am interested in the concept of power and theories about it (soc.psy), I would like to find a specific experimental manipulation of it - and so far I am doing terrible! So,...

02 December 2017 6,806 3 View

Anti-FLAG-tag antibody for flow cytometry in mouse without major background signal issues?

Hi there, Can anybody recommend an antibody that works well to detect FLAG-tagged protein in mice with flow cytometry? I am considering to use FLAG tag for generating a transgenic mouse. HIS tag...

26 May 2016 3,404 4 View

Day care quality: Did anyone work with the MELQO - Measuring Early Learning Quality and Outcomes?

We heard that the official version of the MELQO is not yet ready to use. But we wondered whether maybe someone has a research version of it or some other experiences to share. We would like to...

27 April 2016 9,234 1 View

Antibody doesn't work for proteins of HEK lysate?

Hallo, I am doing some western blot experiments at the moment and have some problems. I transfected a construct in HEK293 cells and loaded a lysate to sds gel and blotted the proteins to...

13 April 2016 3,452 9 View

Too many different values for H2O2 extinction coefficient. Which one is correct?

I am confused with literature data about H2O2 extinction coefficient. I found that the values of H2O2 extinction coefficient depends on wavelength, but in literature, it could be found different...

22 August 2015 8,457 14 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View

Design parameters of L1 frequency Helix antenna?

Could I please get the guidance on Helix antenna operated from 1.5 - 1.8 GHz. I am designing in hfss and not able to cover 1.5 GHz. Could I get papers or suggestions on numbers I use to cover this...

02 February 2021 622 7 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Should I make 2 helical antennas? One for 2.4 GHz and another for 5.8 GHz?

Should I make 2 antennas, one for 2.4 GHz and one for 5.8 GHz? How should I approach the problem if I want to design the antennas which cover 400 and 900 MHz and 2.4 and 5.8 GHz? All together in...

26 January 2021 906 3 View

VSWR is shooting to 2.1 or even 2.3 in UHF dipole antenna, why?

So, I have a design, two dipoles working at UHF and VHF. We fed using a diplexer. I am having difficulties with the testing using network analyzer. VHF passes but UHF, specially at 400 MHZ, the...

26 January 2021 8,743 8 View

Can I simulate Ferrite in HFSS?

I have a design with 2 dipoles working at different frequencies joined in one system and separated by Ferrite. I think, Ferrite was used to minimize the interference between 2 antennas as much as...

25 January 2021 5,918 3 View

Testing of Antennas?

So, I came across, where antennas are being tested in a building ( a plant), where all sorts of big equipment are around and of course the ceiling. lot of multipath which can affect the...

21 January 2021 9,437 3 View