Several years ago I used a goat polyclonal antibody from Boehringer Ingelheim that worked very well, but they are not longer selling this product. Does anyone know which company sells the...
02 March 2013 3,511 1 View
I'm performing transfections with shRNA in cultured cells for specific knock down of a protein of interest. I suspect that knocking down promotes proliferation or maybe by scramble control...
08 September 2012 7,586 31 View
I want to introduce a single mutation by overlap PCR using a pair of 20-mer primers (complements of one another) containing the mutation plus two 5' and 3' end primers. In the first PCR (extension...
08 September 2012 6,797 26 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...
01 March 2021 3,610 1 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...
01 March 2021 9,511 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...
24 February 2021 4,578 4 View
We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...
23 February 2021 1,898 1 View