I try to immunostain serotonin 1A receptors in the rat spinal cord. I have tried antibodies of this kind from several different commercial sources, including Millipore-Chemicon, Abcam and Santa Cruz, but no one seems very specific – each one gave a different staining result. The antibody I’m looking for is the one that stains the neuronal cell bodies but not the axon initial segments. Can anyone give me a recommendation?
See this paper: I obtained good results with a policlonal antibody SantaCruz against 5HT1AR.
J Chem Neuroanat. 2007 Jan;33(1):42-52. Epub 2006 Dec 6.
Monoamine receptors and immature cerebellum cytoarchitecture after cisplatin injury.
Roda E, Avella D, Pisu MB, Bernocchi G.
Dipartimento di Biologia Animale, Laboratorio di Biologia Cellulare e Neurobiologia, Università di Pavia, Italy.
In my hands, there is no good commercial antibody against the 5-HT1A receptor. All the antibodies I have tested gave background in region not known to express the receptor (that can be identified with specific labelled ligand).
In situ hybridization identifies clearly the soma expressing the receptor, See the ref:
Heterogeneous distribution of the serotonin 5-HT(1A) receptor mRNA in chemically identified neurons of the mouse rostral brainstem: Implications for the role of serotonin in the regulation of wakefulness and REM sleep.
Bonnavion P, Bernard JF, Hamon M, Adrien J, Fabre V.
J Comp Neurol. 2010 Jul 15;518(14):2744-70. doi: 10.1002/cne.22331.
Have you tried to use the 24504 Serotonin receptor 1A (HTR1A) antibody of Acris Antibodies?
Thank you all for the very valuable and constructive comments and suggestions. I have tried the following serotonin 1A antibodies: Millipore-Chemicon, AB15350; Abcam, ab44635; Santa Cruz, sc1459 (goat) and sc10801 (rabbit). I didn’t try 24504 from Acris. Is it specific, Luis? Is its specificity strictly validated? Since all the antibodies I have tried gave different results, I’m really concerned about their specificity. Maybe all of them are not specific as Michele said.
I have not tried this acris antibody,but I havve tried five different... Maybe this one works!!! If yes let me know
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