Can anyone recommend a anti-APOBEC1 antibody for immunochemistry?

More Jochen C Meier's questions See All

How do clinical researchers measure transference in psychotherapy patients/clients?

I have a hypothesis that the ceiling effects that appear on measures of the working alliance may be related to positive transference by patients/clients. But when I look into the research...

28 December 2023 4,740 7 View

Which statistical analysis to use for agreement of measurement in count data?

Hello, For my research, I want to know if there is measurement agreement between to tests that I performed. The tests are both performed once, and the outcomes of the tests are count data. I...

02 May 2023 6,019 1 View

Can anyone suggest me a topic for my bachelor thesis in Supply Chain Management (SSCM/Technology/China)?

Hello everyone, I am currently looking for a topic for my bachelor thesis. I would like to combine the two major topics of Sustainable Supply Chain Management and the use of disruptive...

04 October 2021 6,697 5 View

Is there a (dis-)advantage in dicotomizing Likert data?

Given there is some k-step (k=3 or 5 or 7 etc) Likert data with "absolutely no" on one side, "absolutely yes" on the other side, and "neutral" in the middle. A correct way to analyze such...

07 August 2020 1,343 5 View

How to internally link (or cross-ref) ioslides?

Using RStudio and rMarkdown, I have an ioslides presentation (an html file). On one of the slides I want to have a link (cross-ref) to another slide in the same html. I did not find any useful...

28 May 2020 3,008 0 View

Entrepreneurial Orientation Measurement Results?

Hello, I am looking for a review of EO measurement results via the Miller/Covin Slevin scale. I mean the actual values for innovativeness, risk and proactiveness. There are a number of papers who...

18 November 2019 8,753 3 View

Paper describing the shape of an oil droplet on water?

i try to simulate the shape of a 100 microliter olive oil droplet on water. looking at the interfacial tensions of the system (approx. values from varoius papers: water-air 72mN/m, oil-air...

17 November 2019 9,456 3 View

OTUs or ASVs of for microbial network analyses?

Hello! I have bacterial (16s V3-V4) and fungal (ITS2) miseq data from soils collected on different farms where I'm hoping to compare microbial networks in different farming types (conventional and...

23 January 2019 6,914 6 View

Is it correct to call lo(w)ess "non-parametric"?

It's a weighted least-squares polynomial regression, so it's based on assuming normal errors, and the normal probability model is parametric. However, in some statistics book and online statistics...

28 October 2018 3,219 6 View

Amount of secreted protein in HEK293T media?

Hello everyone, i am trying to overexpress a protein in HEK293T cells. I have cloned it into an expression-vector with a strong CMV promotor and transfected the construct into the cells with high...

11 May 2018 721 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View