Is there a (dis-)advantage in dicotomizing Likert data?

Given there is some k-step (k=3 or 5 or 7 etc) Likert data with "absolutely no" on one side, "absolutely yes" on the other side, and "neutral" in the middle. A correct way to analyze such...

07 August 2020 1,107 5 View

Entrepreneurial Orientation Measurement Results?

Hello, I am looking for a review of EO measurement results via the Miller/Covin Slevin scale. I mean the actual values for innovativeness, risk and proactiveness. There are a number of papers who...

18 November 2019 8,474 3 View

Paper describing the shape of an oil droplet on water?

i try to simulate the shape of a 100 microliter olive oil droplet on water. looking at the interfacial tensions of the system (approx. values from varoius papers: water-air 72mN/m, oil-air...

17 November 2019 9,357 3 View

Is it correct to call lo(w)ess "non-parametric"?

It's a weighted least-squares polynomial regression, so it's based on assuming normal errors, and the normal probability model is parametric. However, in some statistics book and online statistics...

28 October 2018 2,985 6 View

Amount of secreted protein in HEK293T media?

Hello everyone, i am trying to overexpress a protein in HEK293T cells. I have cloned it into an expression-vector with a strong CMV promotor and transfected the construct into the cells with high...

11 May 2018 662 3 View

Patlak slopes from dynamic PET data with delayed begin of acquisition?

I'm currently working with pesudo-dynamic PET scans, i.e. data containing sequentially acquired time frames. Using all the time frames I can 'easily' compute the Ki-values (slopes of the...

11 August 2016 4,566 1 View

Are these models equivalent?

27 January 2016 410 7 View

Any freeware tools similar to SPSS?

Are there any good freeware tools for statistical analysis like SPSS or other similar tools? Would also be interested in low-fare tools that are not too expensive

18 January 2016 5,416 3 View

How to calculate the sample size (t-test) when additional data is available to estimate the standard error?

Consider following case: There are k different groups, but only the mean difference between 2 of these groups should be tested (t-test, consider the assumptions are met and alpha and beta are...

14 October 2015 7,922 11 View

Is there any disadvantage to apply post-acquisition filters in ECG, EMG and respiration and no real-time filters?

I would like to record ECG, EMG and respiration (with a chest belt) data in humans and have a general question about the filter settings. The recommended filter settings for my study are ECG 1-35...

02 February 2015 5,253 6 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View