See this link:
http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/mak075bul.pdf
See this link:
http://www.biovision.com/manuals/K661-100.pdf
http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/mak075bul.pdf
http://www.abcam.com/ps/products/65/ab65656/documents/ab65656%20Ascorbic%20Acid%20Assay%20Kit%20Biological%20Samples%20(Website).pdf
http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=8&ved=0CFgQFjAH&url=http%3A%2F%2Fwww.aatestingservice.com%2Fdownloadqualitymenu.php%3Ffilename%3DVitamin%2520C.pdf%26PHPSESSID%3D8282f24001fe3f6a10219d1d95c45473&ei=lHx6U_SqGYmVqAb7iYDYCg&usg=AFQjCNFTwq2adx-Qn2NqACoW_7E57FPgAA&bvm=bv.66917471,d.b2k
standard curve with what kind of sensor? UV-Vis, probably? If that's the case, the molar extinction coefficient might be more useful: Extinction Coefficient: EmM = 7.0 (265 nm, water), 7.5 (245 nm, acid). Taken from: Data for Biochemical Research, 3rd ed., Dawson, R. M. C., et al., Oxford University Press (1986), p. 116.
Although, you should build yourself a calibration curve prior to start your measurements. Ascorbic acid is a piece of cake, just bu sure of preparing your stock solution fresh. At UV-Vis you should have linearity up to at least 200 ppm, which is great.
You can to find more information in these articles:
A novel method for the determination of ascorbic acid and antioxidant capacity in Opuntia ficus indica using in vivomicrodialysis. http://www.sciencedirect.com/science/article/pii/S030881461301385X
Ascorbic Acid, Total Phenol Content and Antioxidant Activity of Fresh Juices of Four Ripe and Unripe Citrus Fruits. http://www.e-journals.in/pdf/v1n2/303-310.pdf
Encapsulation of polyphenolic antioxidants from medicinal plant extracts in alginate–chitosan system enhanced with ascorbic acid by electrostatic extrusion. http://www.sciencedirect.com/science/article/pii/S0963996911001876
Dear Sir,
Agree with Carlos, you should prepare your own ascorbic acid standard curve. It is not difficult to do anyway. Using other's vit.C standard curve may give you inaccurate data because the type of instrument or mechanism of detection may not be the same as what you are using for your sample measurement. The skill of the operator also may affect the accuracy of the data/curve. Besides, you will get more satisfaction and more confident using your own standard curve, more so if you get the curve linearity of 0.99.
All the best.
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