Does anyone have experience using High Molecular Weight DNA Extraction Kits for extracting DNA from plant leaves?

Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...

03 January 2021 9,487 4 View

What is the best way to extract Spike protein from whole cell lysates for ELISA ?

Hello wise scientific community, I am trying to measure the concentration of Spike Protein ( whole protein) expressed by my cell line transfected with a pC DNA 3.1. I can see a nice band by...

17 September 2020 7,265 4 View

Does anyone know about public databases of baseline corticosterone in reptiles?

I'm searching some public databases of baseline corticosterone in reptiles, I know there is a public database of hormones by Vitousek et al. 2018 ( https://hormonebase.org/ ) and has some data...

31 July 2020 540 3 View

IEEE 34 node test feeder in DigSilent PowerFactory?

18 April 2020 1,949 2 View

How to associate parameters to function switch?

I am using comsol to calculate the IV curve using a physical model. The problem is that I am using the switch function to evaluate multiple spectra but I can not associate a temperature for each...

30 June 2019 2,894 1 View

Why in the simulation of a fracture toughness test, the values of stress intensity factor are not uniform along the crack of the specimen?

In the virtual simulation of toughness using a standard steel specimen (A516-Gr.70), the maximum equivalent (Von-Mises) stresses are uniform along the entire length of the crack. However, when the...

21 January 2019 3,694 1 View

Does anyone know an alternative co-stimulation signal to feeder cells or soluble CD28 for CD4 T cells?

I want to evaluate the CD28 loss on CD4 T cells in culture with different stimuli. I used to stimulate this cells with anti CD3/CD28 dynabeads but actually i don't know if it could interfere with...

31 December 2018 2,629 3 View

Can someone tell me where can I find the theory of anxiety postulated by Zung or the original manual?

I need it to write my thesis on anxiety and maternal attitudes in mothers in prision.

11 September 2018 9,452 1 View

How to design qPCR primers specific to cDNA in two different species (mouse & humans)?

Neither my supervisor or I have done qPCR for a number of years and he asked me to look into designing qPCR primers for detecting UCP1 expression in both humans and mice. We would prefer to use a...

10 September 2018 7,542 3 View

How can I understand a SEM micrograph?

Hi, I am trying to identify what phases are present in these pictures, can someone help where can I learn about it? I will really appreciate if someone can give me comments about these pictures.

31 July 2018 3,682 3 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View