I am working on a vector based on pGreen vector which requires pSoup plasmid for replication in Agrobacterium.
I have been trying to assess molecular diversity of rice plants using ISSR primers. I used CTAB method for DNA isolation. I have tried with different reaction mixture conditions and PCR...
06 July 2014 5,973 7 View
I am interested in isolating a gene from arabidopsis whose size is 1.2 Kb. I have synthesized cDNA using oligo dT primers, random primers and gene specific primers. But in none of the samples, I...
06 July 2014 2,853 2 View
I am looking for plasmid isolation for agrobacterim clones for pcr analysis.
11 December 2013 6,240 4 View
I would like to know if any one is currently working on plant viruses of cucurbit crops
03 April 2011 2,949 0 View
03 April 2011 247 0 View
02 March 2021 3,060 3 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View