Vinoth Alphonse

6 Questions 8 Answers 0 Followers

Questions related from Vinoth Alphonse

Why am I having problems with the isolation of a 1.2 Kb gene from Arabidopsis cDNA?

I am interested in isolating a gene from arabidopsis whose size is 1.2 Kb. I have synthesized cDNA using oligo dT primers, random primers and gene specific primers. But in none of the samples, I...

07 July 2014 2,900 2 View

What would be the reason for faint bands in ISSR analysis?

I have been trying to assess molecular diversity of rice plants using ISSR primers. I used CTAB method for DNA isolation. I have tried with different reaction mixture conditions and PCR...

07 July 2014 6,044 7 View

Can anyone provide me with pSoup plasmid?

I am working on a vector based on pGreen vector which requires pSoup plasmid for replication in Agrobacterium.

05 May 2014 9,843 0 View

Can anyone suggest a protocol for plasmid isolation from agrobacterium?

I am looking for plasmid isolation for agrobacterim clones for pcr analysis.

12 December 2013 6,294 4 View

hi!

I would like to know if any one is currently working on plant viruses of cucurbit crops

04 April 2011 302 0 View

hi!

I would like to know if any one is currently working on plant viruses of cucurbit crops

04 April 2011 2,996 0 View