Dear community,
I am planning on conducting experiments for which I need to obtain a plasma-free platelet suspension from an aliquote of a platelet concentrate. Do you now any methodology/protocol that allows for washing without extensive cell activation? I need the cells to be a "functional" as possible.
Yours sincerely,
Michael
Platelet washing is very much needed not only for research purposes but also for preventing some transfusional reactions like anaphylaxis, fever etc. Human platelet-rich plasma(PRP) prepared from blood collected in tri-sodium citrate might face its aggregation owing to lower ionic calcium concentration. To get rid of this, isolation and washing of platelet is done with acid-citrate-dextrose solution and the washed platelet cells are suspended in a buffer solution containing 2mM calcium ion solution.
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