Can anyone provide a protocol for acute hippocampal slice staining?

More Binu Ramachandran's questions See All

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

Request to participate in the survey?

I request your kind attention. I hope you all a happy day and successful days ahead. I am a researcher, studying the adoption factor of HR analytics in Indian industries. For this purpose, I...

16 May 2024 7,754 0 View

Trying to develop MoS2 based conductive threads, but couldn't find the suitable methods for coating?

I am working on conductive threads, trying to coat MoS2 over threads for conductive study. But couldn't find a suitable technique for preparing and coating MoS2 over threads.

12 March 2024 1,751 1 View

Have you noticed any obvious change in student behaviour and learning styles after the widespread use of AI tools?

22 February 2024 7,980 1 View

Please anyone will clear my doubt. In cyclic voltammetry techniques, if the b value is 0.35 should called diffusion controlled process?

Cyclic voltammetry kinetics study

12 December 2023 9,901 2 View

Please suggest the best excitation wavelength I should select for Coffee grounds derived CQDs?

I have got coffee ground waste derived Carbon quantum dots. Herewith I have attached the PL graph I obtained after exciting from 290 nm- 500 nm. The highest intensity excitation peak is observed...

07 October 2023 2,238 0 View

Can you please share a link or a data sheet of Quinine sulfate standard corresponding to m(slope) values when excited at 340 nm wavelength?

Hi, I am trying to find the quantum yield of my synthesized CQD samples, I have excited the samples at 340 nm wavelength, and I am trying to find the mST standard value for Quinine sulfate at 340...

21 August 2023 6,902 1 View

How do i find the degree of crystallinty in this heating curve , am I taking the correct area?

Have I taken my area correctly to find the enthalpy of fusion Hm , so here the percentage crystallinty be Hm/Hm0 or i should consider the peak before the melting peak also and use the Hm-Hc/hm0...

14 August 2023 449 1 View

What are the methods to make hemicellulose chitosan film for food packaging?

methods of making hemicellulose chitosan films

25 July 2023 7,070 1 View

How to get the journal citation report (JCR)?

After acceptance from Benchmarking: an International Journal, I checked for the impact factor on which I can fully trust. But I could not find either impact factor or JCR for 2022 or before. Can...

10 July 2023 7,065 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Barbara Serafini

What kind of staining have you to perform of the slices? Are slices from human brain or animal model? If you write more about your experiments and what you need I will try to help you.

Barbara

Binu Ramachandran

Hi thank you for your response and i would like to immunostain some postsynaptic proteins on acute hippocampal slices from mouse brain .

It is impossible to help if you do not write more info: what kind of tissue? Frozen, fixed, paraffin embedded? Cut with and at what thickness? What kind of protein? What does it mean "acute"? I need more info to try to suggest you a protocol for your staining.