Look at Ishikawa method in the early 80's.

I used a kit from Pierce to produce F(ab)2 fragments from monoclonal and polyclonal human IgGs and it worked very well.

We use papain gel from pierce and followed by Protein A puification. Works very good with all subtypes but not at all for mouse IgG3.

For that we use Genovis Frag ITZ (bit costly but works).

Thanks all. I have already used Pierce kit and paparin, and purified the product on protein A and G, but the purity is not good enough, and the yield is very low. I need a large scale of fab2, and want a protocol successfuly used by someone. my email is [email protected]. thanks a lot.

Hi,

Maybe this application note can be of help for you: http://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1372419082626/litdoc29051520_20130628223222.pdf

Let me know if you need any further assistance, and I can get you in touch with any of our experts at GE Healthcare.

Attached is an old protocol that we have used. I added both a Fab and a Fab2 protocol.

thanks all.

Hi Hongtao Lei,

I'm currently facing the same issue as you had. Low yield and also decreasing in functionality (after purification ). How did you troubleshoot that issue in the end? I would be grateful if you share your experience in this regard.