Can anyone please tell me, for calcium ion assay which dye will be best?

20 September 2021 1 1K Report

For evaluating Apoptosis and measuremenyt of intracellular calcium release, there are several dyes like Fluo-3AM, fluo-4 AM, Fluo-8 AM and calcein AM etc. Among all these which dye will be best and why?

Jeremy Pronchik

Don't forget the Fura dyes too. There is a discussion of them on RG already:

https://www.researchgate.net/post/Which_Ca2_indicator_is_preferred_Fluo-4_or_Fura-21

there may be other discussions on RG if want to do a general search.

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