Hello

I don't have a specific answer for you right now, but if I were you I would go through these attachment to check for a suggestion

Good Luck 

http://qjmed.oxfordjournals.org/content/92/12/711

http://www.scielo.br/scielo.php?pid=S0074-02762004000700006&script=sci_arttext

sorry but, It's not my major.

Any commercial pathology lab can do this test. Ask them to do it for you or get them to show you how.

Hello Vishnu;

First, is it human or animal speciment.

If it is animal: There is no standardize protocol for CK measurement, because protocol might be significantly different and they depends from analysis and condition. Protocols depend from kit to kit. You should use the instructions, from the kit you plan to use.

Human sample: As mentioned above, any pathology lab can do this easily, and you should check with them from protocol, they might vary as well, or if you have a kit, follow the kit instructions.