I am not able to understand what exactly have they done in 'Synthesis of Cds Nanoparticle' part under Materials and methods?
They appear to have used several different type of bacteria to grow CdS nanoparticles. The bacteria in the study are ingesting the Cd and S precursors (CdCl2, and Na2S) from the growth medium and they are excreting CdS particles. Essentially the authors fed the different bacteria Cd and S and the bacteria have produced CdS in their waste. The paper appears to be another successful method of using bacteria to conduct a bio-synthesis of CdS nanoparticles, and the 24 hr time frame seems reasonably quick for a bacterial synthesis.
From the micrographs that are shown in the paper, the particles appear very aggregated, however since they were excreted from a bacteria this shouldn't be unexpected. The authors appear to have successfully synthesized the nanoparticles using the bacteria, however from the UV-Vis data it appears they do not have a way to control the size distribution of the particles which are synthesized, and the band edge absorption peak is rather broad. While this method is an alternative to the traditional high temperature synthesis of CdS, it does not seem to produce the same high quality particles typically seen in the high temperature synthesis.
Thank you, yeah they have used bacteria to synthesize Cds nanoparticles. But however the protocol isn't very clear under the heading 'Synthesis of Cds Nanoparticle' under Materials and Methods. I meant I didn't clearly understand the part after the bacterial biomass and supernatant separation for further experiment. The dilution ratios are not clearly mentioned.
Anyways thanks again Mr Dukes.
Sorry I misunderstood what you asking. It is very likely that centrifugation was used to collect the CdS as it sounded like it was either in soluble or in a colloidal state. Often methanol is used to induce precipitation of CdS (with methanol being added until the particles floculate giving a turbid solution), though it would depend on how the surface is passivated. If the authors did use methanol to precipitate colloidal CdS I feel certain this would damage the bacteria and require a fresh culture for a new synthesis. After they particles were separated they would likely be dispersed in an organic solvent for TEM and other experiments as the paper seems to imply the particles do not disperse well in water. I hope that this was more helpful for you than my first answer. Again, I'm sorry I misunderstood your question the first time.
Oh not a problem Mr Dukes, thank you so much. I will give a try with methanol. This was really helpful. Appreciate your help, thanks again.
Hi.. Miss.Aishwarya go to my page and refer my Biosynthesis of semiconductor nanoparticles paper.. If u have any doubts contact me
Hi, Thanks Mr Rajesh. I will take a look at your paper. Appreciate your help.
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