Can anyone know the Primaquine dose on Plasmodium berghei infected Balb/c mice?
Sorry, I don't know...
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I have performed the Molecular Docking using ADFR suite, I have obtained the files in _out.pdbqt format for ligand docked poses, upon inspection, both visually and reading the pdbqt files of...
08 June 2024 8,130 2 View
I am currently studying index modulation aided NOMA technique but my mind blown up with mathematical expressions. Can someone explain to me these; How Index Modulation aided NOMA (IM-NOMA) works?...
27 April 2024 2,736 1 View
i am working with membrane protein and there is a his tag 4 amino acid above the c terminus (seq HHHHHHDLEY). anti his antibody does not recognise the his tag in western blotting. i was wondering...
14 April 2024 2,675 5 View
I have done the LC-MS result of my complex and its molecular weight is supposed to be 515.5 g/mole. I want someone who is experienced in LC-MS data interpretation. I have attached the picture of...
16 February 2024 382 2 View
quick summary: I am looking for a way to slow down lift-off (1165 or acetone) for a given material system (changing materials to solve the problem is not possible) This is a relatively general...
08 January 2024 8,955 2 View
Dear ResearchGate Support Team, I hope this message finds you well. I am writing to bring to your attention a concerning matter regarding the authorship of certain articles on ResearchGate. It has...
25 November 2023 3,908 1 View
how can we calculate average heterozygocities and unique alleles also how we can compare both populations of different ponds?
21 November 2023 3,238 1 View
i need assistance with few terms your help will be appreciated.
21 November 2023 4,398 1 View
Using machine learning algorithms
26 July 2023 3,025 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View