Gene Cloning: What are the potential problems with doubling up with redundant WPRE and minimal CMV promoter?

From my gene of interest, Sox9, when cut into my recipient plasmid, pEZT-BM, this may include two WPRE regions and CMV promoter. This would take place if I were to cut with KpnI and NotI. As such,...

05 November 2020 8,829 1 View

First time project advice: How to close transcription factors, SOX9 and NFIB, into baculoviral vector, pEZT-BM?

Hi all, I am new here and I am about six weeks in to my first ever research project! (I just came here to kick-start my career as a researcher.) :) The aim of my research project: "The aim...

15 September 2020 9,890 3 View

How to crystallize curcumin from ethanolic turmeric extract?

I'm having difficulty recovering curcumin from my ethanolic extract. I tried reducing its solubility in the solution by slowly adding water, but the solid just crashed out of the solution in very...

03 September 2020 3,020 5 View

Is it natural to observe shifts in spin-orbit splitting values of an element for different stoichiometries or compounds containing that element?

Hello, In XPS measurements, I am observing consistent shifts in spin-orbit splitting values for different oxides of the same base element. I'm being told by many that this is not possible, that...

25 May 2020 4,389 3 View

How to resolve tension between social constructionism and positivist epistemologies within a research paper?

I am planning on interviewing women to get their experiences of health care and analysing it from a social constructionist epistemology. I then want to examine whether the women's constructed...

10 February 2020 2,499 4 View

Has anyone carried out public health expenditure reviews on spending on nutrition in their country ?

If you have carried out national budget analysis for nutrition expenditure I would love to read your research findings

03 October 2019 5,213 2 View

What technique can be used for imaging wet hydrogels?

I'm working on alginate-based hydrogels and I want to observe the microstructures (porosity, roughness, etc.). The hydrogels were produced by gelation in aqueous calcium chloride. SEM is out of...

30 August 2019 2,863 4 View

Does anyone know how and can share how to suspend and spray dry tungsten carbide in an aqueous medium?

I am looking to create a slurry of tungsten carbide to generate press ready powders in order to fully density our laboratory scale small parts for testing. If anyone can share how to suspend WC...

12 June 2019 5,630 3 View

What is the best strategy to launch a technical product that solves an educational problem?

We have designed and launched a research platform that enhances the student - supervisor relationship during a research product. The platform encapsulates the process of conducting and managing...

23 January 2019 6,221 2 View

Are L1 LINEs associated with cancers in humans?

I have read, from an unreliable source, that if it weren't for the presence of L1LINEs in humans, humans would not get cancer. I don't think that could be correct.

04 September 2018 4,475 2 View

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22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

SPSS: A scale with multiple items (7-point Likert) across 4 test conditions. How can I correctly summarize it?

Hello, I am currently writing my master thesis where I have a 2x2 factorial design with one downstream variable (2 independent and 2 dependent variables). I need to conduct a multivariate...

11 February 2021 6,014 4 View

Sample preparation for ceramics i.e. iron silicate (fayalite), iron oxides, alumina, spinel mineral (hercynite)?

Recommendations in preparing ceramics for nanoindentation and in the process minimize introducing stresses from sample preparation i.e. polishing stage.

02 February 2021 6,211 3 View

Protocol for bacterial samples preparation for Scanning Electron Microscopy ?

I want to prepare bacterial samples to observe the bacterial cell lysis by bacteriophage protein (endolysin) through scanning electron microscopy. Please, anyone can share the protocol of...

01 February 2021 1,173 3 View

Sample preparation for SEM observation?

hello RG community, I'm looking for Sample preparation protocole for microbiel cells SEM observation. thank you

01 February 2021 7,355 4 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View

How to prepare an algal sample for FT-IR spectroscopy?

Can we measure the bulk composition of biomolecules present within an algal sample directly by placing its powdered form on a FT-IR spectroscopy stage? Or does it require a particular kind of...

30 January 2021 2,303 4 View