MAD or boxplot to detect outliers in non normally distributed data for latency and distance in animal cognition?

I am studying animal cognition and I have data about animals' latency to approach a cue and animals' distance from the cue in a test. Data are not normally distributed and when I do scatterplots...

04 February 2021 503 3 View

Someone has never seen a small band in control white lane not present in other near lanes?

In some end point PCR, I observ a weak band in white lane (no DNA) not present in any other lane with samples to assay and not according to the expected PCR product. I exclude a contamination of...

16 December 2020 4,011 5 View

Excel: How to maintain original conditional formatting of a cell when it is copied into a new location?

Hi! Question about conditional formatting in excel: I have a table in which in the first column there are species names and in the second column numeric values. I used graded colour scale...

12 August 2020 4,900 2 View

How to change the journal after manuscript acceptance?

Hi everyone, I have an issue and I'm hoping you can give me possible solutions or some advice. I, and two other co-authors, had an accepted paper (since 2019 October) in a great wildlife journal...

17 May 2020 5,513 16 View

What is the role of GABA in a protein purification protocol?

Dear all, I'm looking for Cpf1 purification protocol and I found a protocol in which the authors added GABA to the buffers ( from equilibration till the eluition). I'd like to know if I can...

03 May 2020 7,636 6 View

Which program is best to use for making phylogenetic trees (for beginners)?

Hi everyone, I am a Ph.D. student and work in the field of parasitology. I regularly use molecular techniques and would like to approach phylogenetic trees. More specifically, recently, I have...

08 March 2020 9,610 9 View

Site-directed mutagenesis with Superfi Master Mix, Does anyone use this kit?

08 May 2019 6,498 3 View

How long can DNA from dead parasites be detected in mammal samples by qPCR?

We have detected DNA from a protozoan parasite in lymph nodes of infected mice during 15 days after the infection. Could this DNA be related to live parasites?

04 June 2017 6,496 5 View

Ade4package for Mantel Test: Non convenient dimension In addition: Warning message: In as.dist.default(m2) : non-square matrix

22 May 2017 3,039 1 View

How does k63 ub change upon mg132 treatment?

Hi Guys, I cannot find anything in literature. I have olny found a paper in yeast.. I see that K63 ubiquitination decreases upon mg132 treatment. I would have expected an increase...any cues? Tnx...

03 May 2017 570 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View