Can anyone help with the protocols for determining calcium and protoporphyrin/biliverdin contents in chicken eggshell?

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Nikola Puvača

I hope this can help you.

Adeyemi Adegbenjo

Thank you for your worthwhile input

Paul Pape

We have an article currently in review with the Journal of General Physiology describing a new method for determining the total Ca content in a tissue sample. The first version of this article was very well received and we anticipate it will be accepted and published sometime in early 2015. (Atomic Absorption Spectroscopy (AAS) is another method for measuring total Ca content,) The method described in our article involves homogenizing the tissue in a solution containing the calcium chelator BAPTA which should compete almost all of the Ca in the tissue sample. The total Ca content is determined from the Ca-dependent UV absorbance spectra of BAPTA. I'm not sure it would work for you since I would expect that the Ca in the eggshell would not readily dissolve in an aqueous solution, something that would be needed for the Ca to bind to the BAPTA. If you're interested and think you might be able to get around this problem, I could send you the current version of the manuscript. My e-mail is [email protected].