Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Experiment statistical analysis?

07 February 2021 3,635 3 View

Why Selenium nanoparticles synthesized by PEG 200 could not be scaled up ?

I could not synthesize Selenium nanoparticles by PEG 200 at up-scale ( more than 100 ml) as it got aggregated while at small amounts it works fine , I work with 850 PPM using selenium powder in...

08 January 2021 10,049 1 View

How to read forces from a file and apply them to a specified nodes in ANSYS APDL?

I want to apply different forces for every node. These force values are saved in .txt or .csv file. Can anybody help me to do that using APDL command. I attached Ansys input file for my model and...

15 November 2020 2,883 1 View

Which should be used for Anxiety : unselective beta blocker or B1 Blocker ?

which would be more effective in treatment or protection from Anxiety : unselective beta blocker as Propranolol or selective beta-1 adrenergic receptor antagonist as Bisoprolol ?

15 November 2020 7,994 2 View

How Abaqus can generate lumped mass matrix with non zero values only at the diagonal ?

Hi everybody I generated mass matrix by Abaqus, but it has nonzero off diagonal values. I need to get lumped mass matrix with nonzero values only at the diagonal. In Ansys I can turn Lump on...

28 October 2020 5,960 4 View

No title

I searched a lot for a generalized equation or an algorithm that can be implemented on MATLAB. However, all that I found still theoretical analysis. Can you help with the algorithm or the code for...

24 August 2020 8,117 7 View

How to know coationg efficiency of nanoparticles?

If I'm trying to coat ZnO nanoparticles with Silica, how to know how much of ZnO is coated? (coating efficiency)

29 July 2020 1,813 13 View

Extraction of CoA linked to Acyl chains?

I am in need for a protocol dedicated to the extraction of CoA-acyl chains from plant extracts.

17 May 2020 3,459 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

Consensus sequence from ClustalX?

I am doing multiple sequence alignment to design primer for my gene. Can anyone tell me how to select the consensus sequence after alignment. I am using ClustalX for the alignment.

04 February 2021 8,872 2 View

How to define fuel mixture as input in ansys fluent species transport model ?

I want to define a CO + H2 mixture fuel for a combustor. How can I define this in ansys fluent mixture template ? Normal database does not include this mixture . eg. if I want to define a mixture...

01 February 2021 6,860 2 View

What is the suitable buffer for this protein ?

I am trying to find a suitable buffer for storage of a His-tag purified protein. The amino acid sequence is pasted below. Can anyone help please? MMSFVSLLLV SILFPAIQAK QFTKCELPQV LKDMDGFGGI...

01 February 2021 5,216 4 View

Where can I find the receptor binding domain (amino acid sequence) of ALL pathogenic viruses?

I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?

25 January 2021 3,843 4 View

What is the best software for simulation of flow, sediment transport, and wave combination?

Dear coastal and river engineering researchers, I would appreciate some help from people who may be familiar with Flow, Sediment, and Wave related software with regards to the following: - Is it...

22 January 2021 6,024 14 View

Luciferase assay for miRNA-gene: How to obtain DNA (PCR product) from the mutated primer?

To perform the Luciferase assay for microRNA-gene interaction its necessary a primer (wild-type) to amplificate the specific ligation site and also a mutated primer. But if your primer has a...

19 January 2021 8,568 3 View

What company could perform protein extraction, SDS Page and Western blotting?

Are there companies that do protein extraction, SDS page and Western blotting on tissue samples? I have one target protein and would like to know the concentration in each sample.

19 January 2021 790 4 View