I would like to immobilize fungal cells (Candida and Saccharomyces cerevisiae) onto the surface of glass beads in order to use them, after the immobilization, as the inoculum to obtain a liquid colture.
I have found a procedure that uses Polyethylenimine (PEI), that seems to be what I am looking for but I can't find any information on the kind of PEI that is used.
Here you can find the article that proposed this procedure in the first place (1986) where they used "0.2% PEI solution". In this paper no specifics were given on the kind of PEI used and papers published in the following years were not specific on the PEI used either.
Article Immobilization of Yeast Cells by Adhesion to Glass Surface U...
I am not familiar with PEI and I don't really know what I should look for and there seems to be a whole lot of different options:
https://www.sigmaaldrich.com/IT/it/product/aldrich/408727?gclid=Cj0KCQiAorKfBhC0ARIsAHDzsltJ8AQWxOMmivzrPUsKpZgbdRitWbAy9BL_EmVlcz4RrOqR55RcgvoaAuR6EALw_wcB&gclsrc=aw.ds
Can anyone help?
PEI is a poly cation at pH under 9, silica or quartz surfaces are anionic if treated with even weak bases. So if you wash your surface with pH 8, then expose to PEI at 7-8 the PEI will adhere.
there are a lot of different MW of PEI and also linear vs branched. You might want to test some different ones, but I would think a low to medium MW (5-15 kD) solution of branched PEI will be the easiest.
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