this not related method which i did.I was followed by this method.I need the formula for calculation.
Hydrogen peroxide was assayed according to Yu et al.
(2003) by extracting leaves in potassium phosphate
(K-P) buffer (pH 6.5) (centrifuging at 11 500 × g), then
mixing with TiCl4 in 20 % H2SO4 (v/v). Absorbance was
measured spectrophotometrically at 410 nm.
Actually, I want the mathematical formula for calculating H202 content.
Hello,
for calculation you need to calibrate the instrument. For this, you need analyzed standard samples, for example, diluting a concentration solution of H2O2 and analyzed this standard samples following the same method for real sample, you need a least 3 different standard samples. So, after analyzed in this wavelength the absorbance can be used for build a calibration curve using absorbance(y) versus concentration(x), you can be used a graph program for this, after building the curve you have a equation in this shape: y = ax + b; Whith the absorbance of your real sample can be subistitued and you find the concentration of you sample... After this you must calculating the dilution of you has did.
I hope have helping you.
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