Can anyone help? - FAQS.TIPS

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What should i do to make my sample soluble in HRMS compatible solvent (Acetonitrile, methanol or water)?

I want analyze my sample for HRMS, but my compound is not soluble in methanol, acetonitrile or water. what should i do to make my sample dissolve in any of the above mentioned solvent?

14 February 2024 6,945 1 View

How to increase the ebselen yield from the reaction?

I'm following the same procedure that is given in the research paper but I'm getting very low yield. Kindly suggest some ideas to increase my yield.

20 September 2023 2,293 1 View

Y+ value for sloshing in a rectangular tank?

I am running sloshing simulation in a rectangular tank using ANSYS fluent. reynold's number lie in turbulence and as it is a wall bounded problem I calculated the first cell height of inflation...

10 August 2023 3,764 2 View

How to determine sloshing frequency in ANSYS fluent?

I am running simulation on sloshing of rectangular tank in ANSYS Fluent. I gave sinusoidal base excitation. how to arrive at the value of sloshing frequency?

23 July 2023 541 1 View

How to apply base excitation in ANSYS fluent?

I am running simulation on sloshing of rectangular tank in ANSYS Fluent. I need to give sinusoidal base excitation. I tried giving velocity as transient table boundary condition. somehow I don't...

21 July 2023 2,337 2 View

How long does it take to lyophilize 50 ml liquid sample?

Approximate time taken for lyophilization of 50 ml sample mixed in sterile water. Purpose of lyphilization is HPLC. We have Lyodel lyophilizer in our facility. Model: DPRG-1GH? Thanks!

11 September 2019 2,556 2 View

If we are attempting to do mRNA sequencing(NGS) of a plant whose genome is around 4Gb in size. How many million reads would I get out of RNA seq data?

Since I m new to NGS, I have this basic doubt. Kindly pour your knowledge on this regard.

05 August 2019 4,045 3 View

Can anyone please help on eye tracking system?

Hi, I am trying to understand how to find the XY co-ordinates on screen.I use a single hd camera and one IR light source to make a remote eye tracking system. I am using an open source (Haytham)...

26 July 2016 8,795 7 View

Have any one used Bio Rad turbo transfer for transferring high molecular weight protein around 450kDa?

Hi, I am trying to use fast turbo transfer for transfer of my protein of mol.wt 450kDa. Is it possible to do by turbo transfer? I am trying to get the condition for perlecan western blots. Any...

29 December 2014 6,563 7 View

What is the best way to detect extra cellular proteins like perlecan and nidogen from cultured fibroblast using western blot?

Right now I am using 1% NP40 containing lysis buffer to collect the lysate from rabbit corneal fibroblast cells.

22 September 2014 7,391 4 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 286 0 View

Trial exclusion in eye-tracking data?

Is it reasonable to exclude all trials with a blink or saccade in the 150 ms before stimulus onset? As an alternative, would it be better to exclude blinks (after extending them by about 100 ms...

19 July 2024 3,838 0 View

About exosome isolation?

Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...

01 July 2024 2,558 0 View

As the New JCR impact factor is just released. I need a list of journals which are not tracked for impact factor this year or which are delisted?

As the New JCR impact factor is just released. I need a list of journals which are not tracked for impact factor this year or which are delisted by JCR. Thanks!

24 June 2024 6,586 2 View

Can you share experiences with the modernization of railway passenger traffic in your country?

One of the most pressing open questions for passenger rail transport concerns the sustainability and modernization of infrastructure. This issue encompasses several key aspects: Infrastructure...

22 June 2024 5,589 2 View

Why our eyes can concisely definite left and right, high and low? But cannot near and far?

While analyzing the data of point cloud, i found some strange phenomenon about the precise. But I expect there is no best plan between LiDAR and binocular vision. However, why our eyes more...

21 June 2024 5,269 3 View

What is the best track to have an overview about Electronics and Communication Engineering specializations and the best track in learning ?

I want only to know the several tracks I could get into and the best way to begin.

20 June 2024 8,325 0 View

Ni NTA column nickel leakage problem?

Hi, I am experiencing a nickel leakage from my Cytiva 5mL His Trap column. I think this happened because air went inside the column due to the blockage (or clogging) of the column bed on top....

04 June 2024 3,887 0 View

I am trying to simulate sputtering from a cylindrical target using RSD software. Can anyone help in creating racetrack for cylindrical target?

The target type is titanium; the height is 900 mm, the width is 70 mm, the race track height is 750 mm and the width is 50 mm.

04 June 2024 1,025 1 View

How can I get a list of my publications in Research Gate and the number of Reads and other stats?

I would like to easily track which of my articles are getting reads and which are not without clicking on articles one at a time.

22 May 2024 9,047 3 View

Abirami Muthurathinam

I am working with a single hd camera and light source. Obviously the remote eye tracking. I wanted to know if can we detect people with reduced vision like short sightedness or macular degeneration with this system

Hello Pat,

The pdf content was so useful. I got answers for a lot of questions about remote eye tracking and its calibration. Thank you very much Pat.