I have obsorved the problem while performing the HPLC.as rifabutin was entrapped within the polymer so i was trying to first seperately perform the blank i.e glucan and then our formulation (Polymer to drug ratio was 50:50) mobile phase is Acetonitrile and phosphate buffer(55:45) and Ph of buffer in which formulation was prepared was PBS (7.4) and sodium acetate buffer (5.2) the plane drug peak and the drug inside polymer was quite different in respect to retention time and Area.
So plz suggest some way to make our study easier
glucan microparticles shells are hollow, Porous 2-4 micron in size
To what I have perceived, your drug loaded glucan particles donot release drug in HPLC studies. First, you should check if there is some sort of interaction between glucan and drug using FTIR or NMR. If no interaction, you can also check the compatibility of solvents and suitability of HPLC method for your drug.
Just to check if the problem is with your method, you may evaluate it quickly in vitro. Take given amount of drug loaded glucan particles in a 2 tubes, and add water and HPLC mobiles phase separately. Shake it for 1 hour and check supernatant for drug. If drug is present in water only, the problem is with either selection of mobile phase or method. If drug is present in both water and mobile phase, the problem may with selection of method i.e. HPLC, reverse phase HPLC, HPGC, MS, type of column or flow rate etc.
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