Sample "prep" depends on the actual analysis method used and of course, the goals (what do you wish to analyze for?).
- Without additional info, any responses that provide you with "instructions" without actually knowing what it is you wish to analyze for should be ignored.
Sample preparation must be correctly planned or compounds may be lost during prep, leading to inaccurate conclusions. Once you identify the analysis goals and compounds of interest, a keyword search on the web (e.g. GOOGLE or BING) will provide you with many example application notes and methods to review.
*Please speak with the experienced chromatographer in charge of the analytical instrument at your school for assistance and advice for YOUR sample(s). These complex instruments require many years of training to use as just a basic level, so please have someone with many years of professional training help you with your project.
BTW: The technique is referred to as "HPLC", not "UPLC". Terminology is important in science... **UPLC is a trade-name, brand, used by Waters, the technique is always known as HPLC, or in your case, LC-MS/MS (aka: HPLC-MS-MS).
The protocol will differ according with the type of molecule you want to analyze in the urine.
But most of protocols use solid-phase extraction (SPE) and/or amicon filters
General protocol for urine sample preparation for UPLC-MS/MS:
I prefer to first of all dried sample can be used better. If you can dry then mix it with a solvent system equivalent to a polarity of 2-3%of methanol in chloroform.
Solvent system can be selected with the help of solubility /polarity/nature of the compounds present in the sample.
Yes you may use solvent as reported in previously published research articles.
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