First the dry soil is mixed with CaCO3 and kept 24 hours at room temperature then the sample has been pretreated by dry heat (at 80˚C for 20 minutes), moist heat (at 60˚C for 20 minutes at water bath), sonication and 1.5%( phenol+ saline solution). Then 100 µl aliquot from 10-4, 10-5, 10-6, 10-7, 10-8, 10-9 dilutions were plated on starch casein agar plate containing fluconazole at a concentration 60µg/ml. But there were no single colony rather a cloudy and turbid growth on the entire plate. I have seen and followed many paper but didn’t find any solution. Please give me some suggestions from your experience. Thanks.
Hi,
Please go through these articles.I think these publications will be useful for you.
Article Actinomycetes for Marine Drug Discovery Isolated from Mangro...
Article Antimicrobial activities of actinomycetes isolated from unex...
Article Selective isolation and antagonistic activity of actinomycet...
Article Isolation and Identification of Actinomycetes from Mangrove ...
Article Isolation and screening of Streptomyces sp. from coringa man...
My cordial thanks to Mushtaq Ahmad, Syed Abbas Jafar, and Soumya Mandal for your help.
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