I want to make some gene knockouts in Barley using CRISPR/ Cas9. I want to transform the pre-mature embryo through agrobacterium. Can any one suggest me how to isolate embryo from the small seeds of Barley cv Golden Promise.
Please see the attached file and following reference.
Holme I.B., Brinch-Pedersen H., Lange M., Holm P.B. (2012) Transformation of Barley (Hordeum vulgare L.) byAgrobacterium tumefaciens Infection of In Vitro Cultured Ovules. In: Dunwell J., Wetten A. (eds) Transgenic Plants. Methods in Molecular Biology (Methods and Protocols), vol 847. Humana Press