Ashish Srivastava

10 Questions 8 Answers 0 Followers

Questions related from Ashish Srivastava

Regarding Oxford Nanopore MinION/ Flongle?

Hi All, I am standardizing MinION for virus detection. I observed that the sequence reads improved with the concentration of the input samples in the Device. However, when I looked into the...

15 November 2022 1,987 0 View

Which is a best method to calculate the RFU values (came from fluorometer) in CRIPSR/ Cas based detection?

Dear All, I am having some RFU values of virus infected samples, positive control, negative control and no template control in an experiment. I would like to plot a graph for RFUs. Can someone...

18 October 2022 3,339 0 View

Can Cas13a protein directly purify from PAGE gel, please suggest me the method?

I am willing to Cas13 in vitro. Can it be directly purified from PAGE gel, please suggest me the method.

20 January 2021 1,517 3 View

What methods should I use to show the diversity among viral species?

Genus begomovirus is very diverse group of virus and comprises of many species. If I wish to show the diversity of the begomovirus species in India, what bioinformatics and statistics tools should...

06 April 2018 2,566 2 View

Can anyone help me to get method for premature embryo transformation of Barley?

I want to make some gene knockouts in Barley using CRISPR/ Cas9. I want to transform the pre-mature embryo through agrobacterium. Can any one suggest me how to isolate embryo from the small seeds...

22 February 2018 6,544 2 View

What genome sequence of a begomovirus genome is required to be detected by the viral movement protein? Is it the IR region?

Movement protein in begomoviruses detect the circular viral genome and help its movement from one cell to other. What genome sequence of a begomovirus genome is required to be detected by the...

14 October 2017 4,970 0 View

What genome sequence of a begomovirus genome is required to be detected by the host protein? Is it the IR region?

I was trying to search the literature which may provide the mechanism of action of movement protein of a bipartite begomovirus. Is there any evidence present that can suggest the movement protein...

20 September 2017 9,470 1 View

How to clone 3 DNA fragments sequentially in single vector?

I have 3 synthetic DNA fragments, having different restriction sites. (Fragment1: Spe-I/ Apa-I; Fragment 2: Apa-I/ Eag-I and Fragment 3: Eag-I/ EcoRV cloned into different plasmids. Now I want to...

30 March 2016 2,953 6 View

Can we get desired gene editing using CRISPR/ Cas editing method?

Generally CRISPR/ Cas uses NHEJ for joining DNA fragments. In this method addition or deletion can appear. Is there any method to get desired addition or deletion in all the cells of T1 generation.

14 September 2015 7,793 5 View

Is there any technique that can confirm the high chlorophyll content in necrotic cells compared to healthy?

Respected All, I am facing a problem in an experiment. A necrotic leaf tissue have more chlorophyll content than a healthy one while quantifying with same amount of leaf (same weight). I tried to...

05 April 2015 3,314 4 View