I differentiated MC3T3-E1 cells to osteoblasts by using differentiation kit. I put some vials of differentiated cells in nitrogen tank. Recently, I used 2 vials of same passage number and I saw these little things only in one of my flask, and the other flask was fine.
I put cells from both flasks on my biomaterials and did XTT, and there were no significant difference between data!
Does anyone see anything similar to these in their culture medium?
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