Is Analysis of Similarity (ANOSIM) sensitive to differences in sample size?

Hi there. I want to compare 10 small forest landscapes on which different harvest designs were applied and see how much their attributes (structure, species, soil moisture...) differ. ANOSIM seems...

29 March 2020 5,826 4 View

Is there a way to download all molecules from a published research paper?

Hello, Is there a (preferably free) way to download all molecules (SMILES or SDF) from a published scientific article? Like searching pubchem or chembl or ZINC via DOI or so? I would like to...

05 January 2020 7,542 3 View

How to add a journal ?

Hello, is it possible to add a journal ? (Les Cahiers de Neuropsychologie Clinique) Some of my references can't be complete. Thank you.

08 October 2019 8,622 3 View

What would be an appropriate method to compute the watershed of a lake?

Hi there, I'm wondering what would be the best approach to create the watershed of a lake. I see two solutions here: - Creating the watersheds of all tributaries of the lake and aggregate them,...

02 March 2019 2,527 3 View

Is surface water supply a hydrologic service, a forest service, or a watershed service?

There is no doubt that the access to surface freshwater is an ecosystem service. However, I feel that the many publications on ecosystem services and disservices with respect to the water supply...

07 January 2019 4,166 13 View

What are your tips for obtaining maximum resolution on an agarose gel?

I want to resolve very small deletions on agarose gel. My fragments are ~ 200 bp. What are your tips to get the best possible resolution? Here is what I have already gathered around: - 4%...

18 November 2018 5,740 6 View

What is your favourite long-range PCR enzyme?

I want to amplify a long region of around 20 kb from human genomic DNA. I understand this is around the maximum of what is feasible, but looks possible, at least according to manufacturers'...

17 June 2018 4,218 6 View

I am looking for post-doctoral position that may begin in the fall. Can you help?

I defended my dissertation for the Payson Program for Global Development at Tulane University in May and I am currently working on the required edits. The title of my dissertation is "A...

05 June 2018 3,032 0 View

How to install Neo (Python package for working with electrophysiology) for MAC?

I am wondering if it is possible to install Neo (Python package for working with electrophysiology) http://neo.readthedocs.io/en/0.5.0/ on a Mac. If yes, how to do it?

21 February 2018 2,857 1 View

Anyone using Brookhaven BIC90 for zeta potential measurements. What is the polymer of the Organic Solvent Electrode?

I need to measure zeta potential of dispersions in organic solvent NMP DMF. I can't seem to find the material of the electrode, what polymer and it is compatible with the solvents.

02 January 2018 8,876 2 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

How to find if Qualitative and Quantitative (discrete) variables have a relation?

I'm going to do a brief research about the dependance between the number of cases of Covid-19 and sex( male/female) . One of the requierements is to use inferential statistics to test the...

18 February 2021 7,092 11 View

Is CXCR5 on T cells affected after a cell sorting?

I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...

15 February 2021 8,034 7 View