Can anyone guide me about method to measure the Activity of carbonic anhydras enzyme in plant leaves ?

07 July 2019 1 779 Report

Can anyone guide me about method to measure the Activity of

Carbonic Anhydrase (EC4.2.1.1 CA) enzyme in plant leaves by spectrophotometer ?

Do you want to measure the activity of the enzyme while it is still in the leaves, or are you going to make an extract of the leaves?

There is literature on measurement of CA activity in leaf extracts that should provide guidance. Here are two examples.

Article Carbonic anhydrase assay: Strong inhibition of the leaf enzy...

Article A Modified Assay of Carbonic Anhydrase Activity in Tree Species

You also have to think about which part of the leaf you are investigating, and which subcellular compartment.

Article Plant Carbonic Anhydrases: Structures, Locations, Evolution,...

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