How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Cox regression baseline hazard?

Dear colleagues, I would like to make a mathematical equation of Cox regression model, however I am stuck with baseline hazard. I clearly understand interpretation of coefficients, but I can't...

24 February 2021 6,207 3 View

How to calculate the electric field in the rotating frame (the question in the general relativity)?

In the classical electrodynamics, there is one problem - to find the EM fields created by the charge moving with the constant velocity in the circular orbit or in the helical path (free electrons...

12 January 2021 3,689 1 View

Do you know anything about Elena Bokus, the early 20th Century Architect / Architectural Designer?

The available research of early 20th century history of architecture in North Macedonia, suggests that probably the first female architect / architectural designer was Elena Bokus (born in 1893...

23 December 2020 2,039 2 View

Can you recommend a short and free Deep Learning course that is sufficient to enable implementation on Java?

It would be best if the material is available on the web, a pdf version is also possible. If you still have knowledge of a good video course, please share.

29 November 2020 4,769 2 View

Why the Helmholtz decomposition theorem is not applicable to the electrodynamics?

My question is caused by one curious fact of the electrodynamics. It is commonly accepted opinion that the electromagnetic fields can at least formally be decomposed onto the rotational and...

10 September 2020 8,668 3 View

COS7 cell transfection - do you have a good protocol?

Dear all, lately we ran into trouble with COS7 cell transfection, we get low transfection rate with the protocol we use. Do you have a good transfection protocol? Preferably Calcium transfection...

23 May 2020 842 2 View

Can an electron and a hole have one common coordinate y_0 of the center of the quantum orbit in a two-dimensional semiconductor in a magnetic field?

Can an electron and a hole have one common coordinate y_0 of the center of the quantum orbit in a two-dimensional semiconductor in a magnetic field? (if they have the same or opposite directions...

16 January 2020 5,009 8 View

What is the density of excitons on a two-dimensional semiconductor in an external magnetic (and without magnetic) field?

What is the average density of excitons on a two-dimensional semiconductor in an external magnetic (and without magnetic) field? What is the dependence of this density on the magnetic field B?

12 January 2020 173 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View