I am trying to tune my Sciex 4500 using APCI for the USEPA PAH compound list. I have seen this done across a few applications where they compare ESI, APCI, APPI. They all have said APCI is the best choice. Right now i am trying to tune for the compound Naphthalene and i am just not seeing the Ion.
My mobile phase is 50/50 water and acn at a flow rate of 0.5ml/min. I am infusing a solution at 1ug/ml and i still cant see the ion for naphthalene.
Can anyone give me tips or tricks on how to tune for PAH's using APCI. This is my first time.
Hi,
I am using an ESI source for CID experiment between PAHs and noble gas. In general we prepare a solution of PAHs in dichloromethane (0.5 mM) and after we dilute with a solution of AgNO3 in MeOH (0.5mM) (E Peter Maziarz III, Gary A Baker, Troy D Wood,
Canadian Journal of Chemistry, 2005, 83(11): 1871-1877, 10.1139/v05-195). We inject directly without a chromatographic column.
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