Can anyone give me the methodology to analyse Biotin through HPLC?

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MM J D.C

try this way. with a C8 Column. I send you the reference USP 35 FN 28, pag 1631-1632

Sorry FN 30, 1631-1633 here you can find a methodology with a c18 column

Here you have in pdf version for C18 Column

Biotina for C8 Column

Md Babu Mia

Kindly look at the following papers.

https://www.ncbi.nlm.nih.gov/pubmed/17225595