Rajesh M

6 Questions 11 Answers 0 Followers

Questions related from Rajesh M

What is the proper washing technique for HPLC columns (C 18) after being injected with Poultry feed vitamin premix samples?

I am using C18 250x4.6 mm. After injecting the column with Poultry feed vitamin premix samples, I noticed a high elevation in the column back pressure. My mobile phase is composed of 99% Methanol...

11 November 2016 2,898 5 View

Can anyone suggest which is the best HPLC column for a vitamin analysis?

Which is the best HPLC column for vitamin analysis? Which column contain highest carbon content?

10 October 2014 3,828 4 View

Can someone explain why the pressure variation in Pump A and B (HPLC)?

When using the Acetonitrile (HPLC grade) (100% Pump B) for long time, there is pressure variation in Pump A & B. Now I solving this problem by ultra sonication of Pumb B filter.

09 September 2014 8,397 9 View

Can anyone give me the methodology to analyse Biotin through HPLC?

I need the methodology for analyse the Biotin through HPLC by using the following columns, 1. VP ODS 250 x 4.6mm 2. Phenomenex C18 Luna 5 micron 250x 4.6mm

08 August 2014 1,968 5 View

Is there any method for analyzing the fat and water soluble vitamins by using same mobile phase through Shimpack ODS column?

Now, I am analyzing the vitamins by two methods.

03 March 2014 537 2 View

How do I use the dual column in shimadzu HPLC? I need the method of analysis for water soluble vitamins in HPLC?

I need the procedure

10 October 2013 2,416 0 View