Thank you for your reply. I have selected some where in the stack but after calculation button it has automatically selected its choice. can I have my selection? BTW, I Use ABSnake then the stack is transform in ATTAP.
If I understand that correctly, you have issues with the ROI selection..
You might have to add your ROI to the ROI Manager.. so you select the area and then press CTRL+T -- If that doesn't help, you might want to contact/email the authors of the plugin.. giving the the exact version of ImageJ and describe what you're doing in as much detail as possible.
Yes definitely. Also, I do not know whether I use ABSnake or Spline Snake. I tried to find ROI but I could not fid that.Instead I found ROI manager, that I dont think this is an appropriate one. Can you please send me the link.