hi ankit,

i

m confuse here you want to check genetic stability or genetic fidelity

what i think in your case since you use tissue culture technique i.e micropropagation you need to check genetic fidelity instead of genetic stability.

Since SCOT marker target genic region we prefer SCOT but you can not draw complete conclusion on the basis of SCOT marker only

Extract dna from in vitro generated plants and mother plants. Try different scot primers and select those which produce scorable and reproducible bands. You will have monomorphic banding patterns across all dna samples loaded , be it for mother and regenerants, indicating genetic stability.