Hi everyone, I would like to test a tetramer against HLA-DRB1*04:01 and in the HLA typing data I received, I have some donors who are HLA-DRB1*04:02, DRB1*04:06 etc.. Can anyone explain the detail in these haplotypes and whether a tetramer for DRB1*04:01 is likely to work against DRB1*04:06.. Thanks very much in advance!
Hi Elsa, that is definitely interesting and worth considering.
In terms of what I have, we found HLA-Class II epitopes using overlapping peptides of a viral genome, and I confirmed they are specific to HLA-DRB1*04:01 using ELISpot (using CD8+ T cell depleted sample) and flow cytometry (using peptide stimulation to look for proliferation and IFNy production). So with the data we have generated we are very confident that this epitope is specific and the assays are sensitive. However, we have had limited number of donors with the HLA-DRB1*04:01 allele, but we have subjects with HLA-DRB1*04:02 or 04:06 etc. I mean I appreciate that these mean they are different alleles, but I can't seem to find anything regarding tetramer specificity. My feeling is that the peptide may be presented on the HLA-DRB1*04:02 just like it is presented on HLA-DRB1*04:01 but whether the tetramer will work is a question. We are limited in the frozen PBMCs so I can't just 'give it a go and see what happens' as some would say :)
Hello Elsa,
I haven't tested them on the ELISpot yet unfortunately
Dear Aleks Kamer Guvenel ,
I do not understand your question.
A good T-cell receptor tetramer against DRB1*04:01 should not react with other DRB1*04 alleles like HLA-DRB1*04:02, DRB1*04:06 etc.!
But you must test this!
There could be a crossreactivity!
Best regards,
Thomas
Hello Thomas,
You have answered my question! I was asking whether it should bind but you answered that it shouldn't so thanks very much for your input on the discussion.
Best wishes
Aleks
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