It looks like it would work for DNA. A couple of quick questions: what protein estimation method were you using?  Do you plan to use the DNA for other reactions?

Hi mam, 

              I have plan DNA for DNA laddering technique to check the effect of toxins on murine thymocytes and splenocytes. I did protein estimation by bradford for SDS PAGE. Let me know if you want further information.

Thanks.

I would suggest to use BAC assay (https://www.piercenet.com/instructions/2161296.pdf) instead because the Tris in the solution may interfere with the Coomassie blue. If Tris is necessary for the lysis buffer, I would suggest to use  RIPA buffer (50 mM Tris HCl, pH 8; 150 mM NaCl; 1% NP-40; 0.5% sodium deoxycholate; 0.1% SDS, with supplement of of 10 to 20 mM EDTA to prevent DNA degradation) for better nucleus lysis. If not, I would switch to PBS based lysis buffer. On a side-note, if western blot analyses is planed for further experiments, I would be careful with the EDTA, because sometimes it may affect the antibody interacting with the protein. 

Thanks for your suggestion.