Anoop Kumar

4 Questions 10 Answers 0 Followers

Questions related from Anoop Kumar

Can anyone direct me regarding composition of cell lysis buffer for DNA and protein estimation in murine thymocytes and splenocytes?

For protein estimation, I have used the following composition of cell lysis buffer: EDTA (20mM), Tris HCL (10mM) and Triton X-100 (0.5%). Can I use the same composition for DNA estimation or Is it...

19 January 2015 2,850 4 View

What is the best way to perform proper gating in flow data?

My question is regarding the phenotyping of CD4+ and CD8+ cells of murine thymocytes. Is there is any rule for gating? Thanks and regards: Anoop

15 January 2015 290 3 View

How are the environmental toxins are presented to the T cells?

Is it is same way as that of pathogens is presented to T cells i.e. binding with Major histo-compatibility complex (MHC) or Is it different? Thanks and regards Anoop

06 January 2015 6,615 5 View

Is it necessary to perform DNA laddering, if I already checked the percentage of apoptotic cells by cell cycle analysis (flow cytometery)?

29 December 2014 7,148 2 View