Can anyone advise me on the use of LaCl3 as a Ca2+ ion channel inhibitor to study Arabidopsis primary root?

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Kenji Shoji

Dissolve 10mM of LaCl3 in water, add 10µL for 100µM to inhibit Ca2+ ionic channels. Verify again your pH. Make sure that your media does not contain phosphate buffers to avoid precipitation, use instead TRIS or HEPES buffered solution.

Joanna Kacprzyk

Hi Keli, what concentration of LaCl3 are you using?

Keli Peng

Hi Joanna， I set a gradient from 0 to 200μM.

Hi Kenji, I use KOH to adjust pH instead of phosphate buffers. Maybe it's because that I didn't add MES to steady the pH of medium. And I will also try Tris and HEPES to adjust pH. Thanks for your advice!