I need to stain B-Raf in PFA-fixed cells and tissue explants
Any luck on this question? I could use a good one, too :)
Which is the best Antibody to perform IP of endogenous mouse p300 IP. Please provide a figure or a citation in your answer. Thank you!
24 September 2019 8,612 2 View
08 September 2019 4,324 2 View
In my particular case I observed an energy-dependent mechanism in my clone of interest also when incubating the cells at 4 °C (30min) as a control. The experiment was still done several times. I...
09 October 2013 9,848 1 View
Can anyone recommend a method to isolate early endosomes from animal tissue without using ultracentrifugation?
11 December 2012 6,847 4 View
What is the best method to quantify early endosomes in cell lines and/or animal tissue. Is the total amount of EEA1 and RAB5 in addition to quantification of EEA1+ vesicles from immunoflurescence...
11 December 2012 4,233 9 View
04 December 2012 3,590 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...
30 July 2024 9,806 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View