I want to do protoplast fusion, for that I need to do haploidization.
Can anybody tell me the protocol of fungal haploidization?
haploidization : after protoplast fusion fusants can be transfer on regenation media containing benomyl this is one of the very common method to do haploidization .
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
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kindly reply me. Thanking you in advance.
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