Murine protein expressed in HEK293 cells used for aspirations in mouse model ?

I am planning to express a murine protein X with a Myc tag in HEK293F cell line - purify the protein - check the purity of the protein - use that protein - X with Myc tag to aspirate mouse. Thus,...

12 February 2020 4,483 1 View

How to get an Asymptote from multiple coordinates obtained from Machine Learning ?

I want know about an algorithm or formula to find the asymptote from coordinates obtained from Machine Learning. Like the ML will always give points precise & closer to the Asymptote if I ran it,...

11 October 2019 1,025 2 View

I have completed my bachelor's in Electrical Engineering and I am looking forward to start learning more about smart grid .From where should I start.?

I want to learn about smart grid and simulating a smart grid network and also the data analytics used in smart grid network .

23 May 2018 6,165 3 View

Has Any One Tried 365nm Fixed Wavelength LED 10 Watts?

29 September 2016 8,339 3 View

Does anyone have experience working with Injectable Cyanocobalamin in rats?

15 September 2014 1,045 4 View

When I tried to precipitate my protein with acetone or TCA, it did not precipitate. But when I mix it with water it precipitated - any thoughts?

My protein is acid extracted. I need to know the mechanism behind it.

29 May 2014 6,787 3 View

Free software to conduct an online quiz?

From the last few months, I am trying to generate an online Quiz for my student, I have prepared some of them also, but find two difficulties: 1. Some students can't open the exam in their...

24 December 2013 2,307 3 View

Shortcut key to merge some cells in table & to split the same!

Can anybody suggest the shortcut keys for Merging & splitting the cell in MS word & MS Excel?

05 October 2013 9,215 13 View

What is the ideal dilution for using the Primary and Secondary Antibody Concentration for 6x HIS tag : Protein?

The 1:2000 for primary and 1:20000 for secondary gives a very dark background .

22 May 2013 4,813 3 View

What if the restriction digest pattern of plasmid midiprep varies from the same plasmid's miniprep?

I normally inoculate 5 - 7 colonies from selective antibiotic LB plate after transformation in the liquid LB with the same concentration of the same antibiotic. The next day, I do plasmid miniprep...

27 February 2013 4,896 12 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How can misfit between business requirents and ERP system business process be reduced?

One main reason for failure of most ERP implementations is the misfit between business requirents and ERP system business process be reduced. How can the misfit between business requirents and ERP...

22 February 2021 5,709 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

How to perform Kaplan-meier survival analysis in SPSS?

Hi everyone, This is my first time of attempting to run survival analysis. I have a data on patients with End-stage Kidney Diseases (ESKD). I would want to run survival probability by treatment...

16 February 2021 2,647 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Hello .Ineed a researchs about systolic heart failure any one can help me ?

Am a master student .I need an information about this case to write my thesis?

09 February 2021 9,706 2 View

Is this grounds for theophylline toxicity even though her plasma is within the therapeutic range? if it is, how is that possible?

A patient was given 300 mg Theophylline taken twice a day for the treatment of her bronchial asthma and has a positive clinical response. She has been compliant in taking her medication however,...

09 February 2021 3,489 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View