ELISA against plant viruses?

We are doing DAC ELISA against plant viruses. I have following queries. Controls that we are using are as follows. Buffer control, Healthy leaf samples, disease infected sample (+ve sample), and...

14 October 2020 1,627 2 View

How to design a circuit for Reconfigurable antenna using PIN diode in hardware?

I have simulated the reconfigurable antenna using PIN diodes using HFSS software.Now for the hardware implementation i am not getting how to give current to the diode. Should i use RLC circuit to...

18 December 2019 6,702 3 View

Can anyone explain how to synthesise t-phase zirconia without calcination or at lower temperature?

I am working on zirconia nanoparticle synthesis, can anyone help me how to synthesis t-phase zirconia at lower temperatures or without calcination?

10 November 2013 5,276 3 View

Can we selectively promote protein crystallization against salt crystallization without a complete change of the crystallization condition?

Consider a crystallization condition that readily gives salt crystals on addition of protein. Is there a logical way to increase the probability of protein crystallization against the undesired...

02 April 2013 853 3 View

Can a protein crystallize after 2 months of its introduction to a particular well condition?

I was recently trying crystallization trials of my protein sample in a 96-well Corning plate. In one well of an experiment, some phase separations emerged after the 4th day of dispensing the...

16 January 2013 9,761 25 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

How to find orthotropic material properties?

I am doing an impact analysis of bullet on a plate. But for that simulation, I require orthotropic properties of materials like PPS and PEEK which are Unidirectional type. How to find them?...

16 February 2021 8,775 2 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

How to purify cycle sequencing PCR reaction?

i am purifying cycle sequencing PCR reaction. i have tried multiple methods given in BDT manual (provided with the instrument) .everytime i used 96 well reaction plate for this purpose. 7 wells...

14 February 2021 8,534 2 View

How do I determine temperature of ethanol during a reflux?

04 February 2021 6,673 4 View