Hello, elution buffer composition depends which method you really use.

See attached info as example.

Good luck.

There are lots of different lipases existing. What kind of lipase are you working with? Do you know anything about a possible pH optimum?

Need to know the pH you are working with. Moistly phosphate buffers do the job if the pH matches.

Hello, elution buffer composition depends which method you really use.

See attached info as example.

Good luck.

Thanks a lot for your answers. I work with pH7

Ok, you are at pH 7.0, which specific Chromatography Mode? IEX, HIC, Affinity, etc.?