Can anybody please provide me neomycin resistance gene sequence or primers for neo gene incorporated in HEK293T cells?

12 November 2015 1 2K Report

I am trying to develop stable cell lines for my desired protein in HEK293 cells through G418 selection. However, HEK293 cells are not getting killed by G418 even at high conc (1000ug/ul). Thus, I would like to look for whether my cells already possess a neomycin resistance gene (neo phosphotransferase) or not? So please help me by providing the sequence or primers for neomycin resistance gene?

Thanks

Sabine Strehl

Read this thread and you can spare yourself the PCR; but you have to clone another resistance cassette into your vector...

https://www.researchgate.net/post/HEK293_or_293T_for_stable_line_selections_with_Geneticin

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