Abdul Mohin Sajib

3 Questions 2 Answers 0 Followers

Questions related from Abdul Mohin Sajib

Have you used 32 Kb plasmid and Superfect Transfection reagent?

Do anyone have any experience with transfecting large size plasmid ( around 32 kb, viral plasmid) with Superfect or Polyfect transfection reagent? What DNA to Superfect reagent ratio to follow?

12 June 2018 6,987 3 View

Can anybody please provide me neomycin resistance gene sequence or primers for neo gene incorporated in HEK293T cells?

I am trying to develop stable cell lines for my desired protein in HEK293 cells through G418 selection. However, HEK293 cells are not getting killed by G418 even at high conc (1000ug/ul). Thus, I...

12 November 2015 2,302 1 View

Can anybody suggest a cheap 6his tagged protein that i can use for western blot?

I am doing western blot and looking to see band for my his tagged protein...

15 June 2015 4,021 2 View